anti human cd40 (Bio X Cell)
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Bio X Cell
anti human cd40
Anti Human Cd40, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+human+cd40/InVivoMAb+anti-human+CD40/pmc13041753-242-25-29
Average 94 stars, based on 29 article reviews
Anti Human Cd40, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+human+cd40/InVivoMAb+anti-human+CD40/pmc13041753-242-25-29
Average 94 stars, based on 29 article reviews
anti human cd40 - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Inflammatory arthritis irAE may represent a unique autoimmune disease primarily driven by T cells but likely not autoantibodies"
Article Title: Inflammatory arthritis irAE may represent a unique autoimmune disease primarily driven by T cells but likely not autoantibodies
Journal: Science Advances
doi: 10.1126/sciadv.aea4262
Figure Legend Snippet: ( A to C ) Human naïve B cells were isolated from PBMCs and cultured under indicated conditions for 7 days; pembrolizumab or isotype control IgG4 (IgG4 Iso) was added on day 2; n = 8. (A) Expression of CD38 and CD27 on B cells. Right: Percentages of CD27 + CD38 − and CD27 + CD38 + B cells. (B) Expression of CD138 on CD27 + CD38 + B cells. Right: Percentage of CD138 + CD27 + CD38 + B cells. (C) Different immunoglobulin isotypes were measured in the culture supernatant of (A) and (B) by multiplex assay. ( D to F ) B cells from Humanized PD-1 (HuPD-1) mice were isolated and cultured with LPS, IL-4, BAFF, or ODN 2006, anti-IgM, IL-21, IL-4 or R848, anti-IgM, anti-CD40, IL-21, and IFN-γ for 3 days; pembrolizumab or isotype control was added on day 1. (D) Expression of IgG2c on activated B cells. FSC-H, Forward scatter height. Right: Percentage of IgG2c + B cells; n = 5. (E) Expression of IgG1 on activated B cells. Right: Percentage of IgG1 + B cells; n = 5. (F) Different immunoglobulin isotypes in the supernatant were measured by multiplex assay; n = 5. Data in graphs represent mean ± SEM. Significance was tested by two-way ANOVA.
Techniques Used: Isolation, Cell Culture, Control, Expressing, Multiplex Assay
![( A to F ) Bead-based multiplex assays were used to measure plasma concentration of IL-6, and IL-12p70 (A); TNF-α, IFN-γ, and IL-1β [B; HC ( n = 19), irAE ( n = 34), RAC ( n = 45), and ICI ( n = 9)]; IP-10 (CXCL10), CXCL11, and CXCL9 (C; HC, n = 17; irAE, n = 33; RAC, n = 46; ICI, n = 17); CCL20 (D); CX3CL1 (E); and CCL2 (F). ( G to J ) Human naïve B cells were isolated and cultured with anti–human CD40 (0.5 μg/ml), anti–human Ig (M + G + A) (2.5 μg/ml), and rhIL-21 (20 ng/ml) with IFN-α (100 ng/ml), IL-6 (100 ng/ml), IL-12 (100 ng/ml), control, or the combination of IFN-α, IL-6, and IL-12 for 7 days. Cells and culture supernatants were analyzed. (G) Representative flow plot of CD38 and CD138 expression on CD27 hi CD38 hi ASCs. Right: A summary of the percentage of CD138 + ASCs; n = 6. (H) Expression of CD11c and CD27 on CD27 − IgD − B cells. Right: Percentage of CD11c + IgD − CD27 − B cells; n = 6. (I) Expression of active-caspase-3 in B cells. Right: Percentage of active-caspase-3 + B cells from different groups; n = 3. (J) Different immunoglobulin isotype levels in the culture supernatants from (G) to (H) were measured by the multiplex assay; n = 6. Data in graphs represent mean ± SEM. Significance was tested by one-way ANOVA [(A) to (I)] and paired Student’s t test (J). [(A) to (F)] ICI, ICI control.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1753/pmc13041753/pmc13041753__sciadv.aea4262-f5.jpg "... B cells were isolated and cultured with anti–human CD40 (0.5 μg/ml), anti–human Ig (M + G + ...")
Figure Legend Snippet: ( A to F ) Bead-based multiplex assays were used to measure plasma concentration of IL-6, and IL-12p70 (A); TNF-α, IFN-γ, and IL-1β [B; HC ( n = 19), irAE ( n = 34), RAC ( n = 45), and ICI ( n = 9)]; IP-10 (CXCL10), CXCL11, and CXCL9 (C; HC, n = 17; irAE, n = 33; RAC, n = 46; ICI, n = 17); CCL20 (D); CX3CL1 (E); and CCL2 (F). ( G to J ) Human naïve B cells were isolated and cultured with anti–human CD40 (0.5 μg/ml), anti–human Ig (M + G + A) (2.5 μg/ml), and rhIL-21 (20 ng/ml) with IFN-α (100 ng/ml), IL-6 (100 ng/ml), IL-12 (100 ng/ml), control, or the combination of IFN-α, IL-6, and IL-12 for 7 days. Cells and culture supernatants were analyzed. (G) Representative flow plot of CD38 and CD138 expression on CD27 hi CD38 hi ASCs. Right: A summary of the percentage of CD138 + ASCs; n = 6. (H) Expression of CD11c and CD27 on CD27 − IgD − B cells. Right: Percentage of CD11c + IgD − CD27 − B cells; n = 6. (I) Expression of active-caspase-3 in B cells. Right: Percentage of active-caspase-3 + B cells from different groups; n = 3. (J) Different immunoglobulin isotype levels in the culture supernatants from (G) to (H) were measured by the multiplex assay; n = 6. Data in graphs represent mean ± SEM. Significance was tested by one-way ANOVA [(A) to (I)] and paired Student’s t test (J). [(A) to (F)] ICI, ICI control.
Techniques Used: Multiplex Assay, Clinical Proteomics, Concentration Assay, Isolation, Cell Culture, Control, Expressing
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other:Article Title: Peptidylarginine deiminase 2 citrullinates MZB1 and promotes the secretion of IgM and IgA. Article Snippet: IL-2 (20U/mL) (NIH BRB), IL-10 (50ng/mL) (#571004, BioLegend), IL-15 (10ng/mL) (#570304, BioLegend), CpG ODN 2006 (10μg/mL) (ODN-2006, InvivoGen, San Diego, CA), human insulin (5 ug/ml) (Sigma #PHR8925) anti-polyhistidine tag antibody (5μg/mL) (MAB3834, Sigma-Aldrich), and histidine-tagged soluble CD40L (50ng/mL) (#32047, Cayman Chemical, Ann Arbor, MI), and AFM-30a (CAY10723, Cayman Chemical) were added after plating. Article Title: Inflammatory arthritis irAE may represent a unique autoimmune disease primarily driven by T cells but likely not autoantibodies. Article Snippet: Surface molecules, including IgD, CD86, CD138, CD27, CD38, CD11c, and PD- 1, were examined by flow cytometry, and the supernatant was used to measure immunoglobulin levels (LEGENDplex Human Immunoglobulin Isotyping Panel, BioLegend). Purification:Article Title: scDrugPrio: a framework for the analysis of single-cell transcriptomics to address multiple problems in precision medicine in immune-mediated inflammatory diseases Article Snippet: Human naïve B-cells were subsequently preenriched by MACS sorting using a B-Cell Isolation Kit II (Miltenyi) and further purified by flow cytometric sorting of CD19 + CD27 − B-cells. .. Purified human naïve B-cells were cultured in 96-well plates in the presence of AffiniPure F(ab') 2 Fragment goat anti-human IgG + IgM (5 μg/mL, CAT: 109–006-127, Jackson ImmunoResearch), Cell Culture:Article Title: scDrugPrio: a framework for the analysis of single-cell transcriptomics to address multiple problems in precision medicine in immune-mediated inflammatory diseases Article Snippet: Human naïve B-cells were subsequently preenriched by MACS sorting using a B-Cell Isolation Kit II (Miltenyi) and further purified by flow cytometric sorting of CD19 + CD27 − B-cells. .. Purified human naïve B-cells were cultured in 96-well plates in the presence of AffiniPure F(ab') 2 Fragment goat anti-human IgG + IgM (5 μg/mL, CAT: 109–006-127, Jackson ImmunoResearch), Recombinant:Article Title: Treating autoimmune disease Article Snippet: Palmitoleic acid (NU-CHEK PREP, INC), palmitic acid (NU-CHEK PREP, INC), stearic acid (NU-CHEK PREP, INC) were conjugated with fatty acid free BSA (Sigma-Aldrich) as described elsewhere (Wang et al., Immunity 35:871-882 (2011)). .. Human B cells were activated with CpG OND2006 (2.5 μM; IDT), recombinant human cytokine IL-10 (50 μg/mL; Peprotech), IL-15 (10 ng/mL; Peprotech), IL-4 (10 ng/mL; Biolegend), IL-2 (10 unit/mL) and |
